Vol. 2, Issue 6 (2017)
Phytochemical screening and antioxidant activity of extracts of the leaf and stem of Achillea millefolium
Author(s): Mohd Yaseen, Mudasar Ahmad, Tareq A. Wani, Manzoor Ahmad, B. A Gani, Rashida Qureshi
Abstract: Achillea millefolium L. sensu lato (yarrow) is the best-known species of the genus Achillea due to numerous medicinal applications both in folk and conventional medicine. It is also an important medicinal plant found in Kashmir. The present study was aimed to evaluate the phytochemical constituents and antioxidant activity of leaf and stem extracts of Achillea millefolium. Antioxidant activity was carried out by using 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) and Nitric oxide assay. The phytochemical screening of leaves and stems of Achillea millefolium revealed the presence of phenols, steroids, tannins, saponins and alkaloids in the hydroalcohol extracts. The percentage yield of hydroalcohol extract of the leaf of Achillea millefolium was higher (13.55) than that of the stem. Also quantitative analysis showed that percentage of phenols was higher (17.47) in the leaf extract. The results of DPPH scavenging activity for leaf hydroalcohol showed (14.87%) and stem hydroalcohol showed (12.95%) inhibition. When compared to standard the leaf hydroalcohol showed better DPPH scavenging activity (14.87%). The percentage antioxidant activity is high in leaf extract than stem extract and in Nitric oxide assay the percentage inhibition was also higher than the stem extract when compared to the Ascorbic acid (standard). It indicates the plant has the potency of scavenging free radicals and it may provide leads in the ongoing search for natural antioxidants from various medicinal plants to be used in treating diseases related to free radical reactions.