In this study, the Natural radioactivity concentrations of tobacco cultivated field samples of Dindigul and Erode districts (23 stations) are measured. The Gross Alpha and Gross Beta radioactivity concentrations are investigated in the period of November 2012 to January 2013. By using an alpha counter (SP 647A) with Zns (Ag), the gross-alpha and gross-beta radioactivity of soil samples is measured. The gross-alpha and gross-beta activity concentrations in soil samples varied from 0.25 to 1.20mBq/g and 1.28 to 6.17mBq/g are observed. These results provide the baseline data for the further studies about daughter radionuclides in selected 23 stations.