A four coordinated square planar copper (II) complex formulated as [Cu (PDTC)₂] (1) (PDTC = Pyrrolidine dithiocarbamate) was synthesized and characterized by elemental, physico-chemical and spectroscopic methods. The electronic structure and spectral properties of the PDTC and complex 1 has been explained by DFT and TDDFT calculations. The lower HOMO-LUMO gap of PDTC indicating molecule requires small excitation energy.The interactions of copper (II) complex towards bovine serum albumin (BSA) were examined with the help of absorption and fluorescence spectroscopic tools. An antibacterial activity of the complex was studied by agar disc diffusion method and minimum inhibitory concentration (MIC) strain against Escherichia coli, Vibrio cholerae, Streptococcus pneumonia and Bacillus cereus. The complex exhibited significant activities against one gram-positive (S. pneumoniae) and one gram-negetive bacteria (E. coli).