Present investigation has been conducted on Amara pond (Raipur Karchuliyan) Rewa of Madhya Pradesh with special reference to its zooplankton diversity in relation physico-chemical characteristics. A total of 14 species of protozoa, 02 species of Porifera, 02 species of Coelenterata, 01 species of Platyhelminthes, 23 species of Rotifera, 05 species of Ostracoda, 02 species of Copepoda, 01 species of Harpacticoida, 5 species of Cladocera, in all 55 species of zooplankton were identified from pond in the year 2014-2015. The investigation on physicochemical characteristics at different sites revealed its alkaline nature, suitable for aquaculture practices. Significant site variations have been recorded due to the interference of sewage and agricultural wastes. Among all the zooplankton groups, Rotifera recorded dominance. Maximum diversity of zooplankton population was recorded at macrophytic sites during summer season.