The present investigation was intended to devise a cost effective protocol for in vitro propagation of important medicinal plant Adhatoda vasica Nees. Thus, the in depth cost analysis of both protocols viz. conventional tissue culture method using MS media and modified protocol using modified tissue culture media/ cost effective media were taken into consideration. The different parameters that were studied here are: cost of electricity, cost of media and cost of other important ingredients. Thereafter, the comparative assessment of both the protocols was made. It was found that the modified method was very much cost effective in terms of cost per plantlet.