There are wide varieties of plants which are naturally available in our environment and its nutritional values are still unknown to us. Psidium guajava is one such plant; its nutrient values are still unknown. So the present study deals with the biochemical and nutritive analysis of Psidium guajava L. The leaves of Psidium guajava were collected from Kannur District, Kerala. The leaves were shade dried and powered. The macronutrients and micronutrients were analysed by following standard methods. Thebiochemical studies reveals that the presence of carbohydrate, protein, starch and amino acid in very less amount. It is also estimated that guava leaves are the good source of vitamins such as Vitamin C and Vitamin B, Minerals such as Calcium, Magnesium, Phosphorus and Iron. The results of comparison between the guava leaf and fruit based on concentration of micronutrients reveals that the leaf has more concentration in Vitamin B, Calcium, Magnesium, Phosphorus and Iron, but the concentration of Vitamin C and Potassium were higher in fruit. Hence the study reveals that Psidium guajava leaves rich in nutrients and can be added in our day to day life.