Antidermatophytic activity Aristolochia bracteolata plant extracts using and isolated bioactive compound from plant extract. The using two different solvents plant extract of Aristolochia bracteolata leaf completely prevented growth of tested dermatophyte species with agar well diffusion method. The preliminary phytochemical screening indicated the presence of alkaloids, flavonoids, saponins, phenol, phytosterol, tannin, carbohydrate and glycosides. Thin Layer Chromatography (TLC) and HPTLC High Performance Thin Layer Chromatography studies indicate the bioactive compound of the plant. The plant Leaf include the Aristolochic acid and Acetylenic acid and flavonoids. Bioactive components and functional groups present in the plant leaf of Aristolochia bracteolata. FT-IR study revealed the presence of various functional groups related to phenols, alcohols, alkenes and Alkyl halides. The twenty-two components identified by GC-MS analysis. The plant extract showed inhibitory activity against dermatophytes genera Trichophyton and Microsporum. This investigation it can conclude the Aristolochia bracteolata for alternative of treatment for dermatophytic infection in traditional and current medicinal uses.