According to Bahadur and Ranganayaki the earliest living cells was very simple in structure and were full of the properties of biological order.

Bahadur in 1967 suggested that the way in which the systems organize themselves with properties of biological order is very important feature of the study of origin of life. Jeewanu, the autopoetic eukaryotes were prepared by Bahadur in 1967.

It was observed that when a mixture of Jeewanu and water is exposed to sunlight, water is split up into H2 and O2.But due to the nitrogenase like activity of Jeewanu, the produced hydrogen is utilized in the fixation of nitrogen.

The hydrogen produced by the splitting of water is also utilized in the reduction of inorganic carbon.

Khare prepared boron molybdenum Jeewanu in 1989 which showed significant water splitting.

The presence of certain transitional metal ions e.g. Mn, Co, Zn, Cu and Fe individually in the parental environmental medium of molybdenum Jeewanu activated the water splitting ability of molybdenum Jeewanu.

An attempt has been made in this paper to study the effect of addition varying concentrations of ZnSO4 to the PEM on the morphological properties of BMJ24 Jeewanu prepared both under oxygenic (PUOC) and anoxygenic conditions (PUAC).