The character of the environment is a degenerating due to the accumulation of several pollutants either direct or in indirect way, which at last causes several unwanted effects on organisms in general and particularly on human beings. The heavy metal lead nitrate is one of the important aquatic toxic pollutants and is ubiquitous trace metal. In the present investigation, an attempt was made to assess the effect of lead nitrate on biochemical parameters in the muscles and liver tissue of Labeo rohita fingerlings. The fish were exposed to a sub-lethal dose 1.56 mg L-1 (1/25th of 96 hr LC50 value of lead nitrate for a period 60 days. At the end of each exposure period, the level of protein, lipid and glycogen were analyzed in selected tissues. Various alteration accures in tissues like muscle and liver tissues. A significant decrease (P<0.01 and P<0.05) was noticed in all the biochemical parameters studied in treated groups when compared to the control.