A new mixed ligand complex has been prepared by reaction of chloride salts of Mn (II) with Shiff base 2- ((E)-4- (E)-phenal-diazenyl) phenol [PDIMP= (HL1)] and (Z)-2- ((P-Tolylimino) methyl) phenol [TIMP= (HL2)]. Both the ligand/complexes were characterized on the basis of microanalysis, melting point, conductivity, solubitity, determinating of the metal contact M%, IR, and UV/VIS spectral studies. The schiff bases acts as a monobasic bidentate ligands, coordinating through deprotonated phenolic oxygen and azomethine nitrogen atoms. The complexes are non-electrolytic in DMSO. From the analytical and spectral data the stoichiometry of these complexe has been found to be [M (L1) (L2) (H2O)2]. Where M=Ni (II). The electronic absorption spectra and magnetic susceptibility measurements suggest the square planer geometry for the Ni (II) ion. The in vitro antibacterial activities of the complex was test using number of bacteria species such as Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Streptocuccus pyogenes, and Basillus subtilis. The Schiff bases acts as a monobasic bidentate ligands, coordinated phenolic oxygen and azomethine nitrogen atoms.