A simple, rapid and sensitive RP-HPLC and method was developed and validated for estimation of Ezetimibe by using in bulk and tablet dosage form. Chromatography was carried out by using C18,5µ (250×4.6) mm phenomenex column as a stationary phase with mobile phase containing a mixture of potassium dihydrogen phosphate and disodium hydrogen phosphate (Ph-7): Acetonitrile in the ratio of 25:75 v/v as mobile phase. The flow rate was 1ml/min. The effluent was monitored at 232nm with the retention time 3.8 mins of drug. Calibration curve was plotted within the range of 6-10µg/ml for ezetimibe and correlation was found to be 0.999. The accuracy range was found to be 98-102%. The %RSD values for all parameters was found to be 2 for. The proposed method can be useful for routine determination of Ezetimibe in pharmaceutical dosage form and the method is validated according to ICH guidelines.