The Yba₂CU₃O₇ (YBCO) HTSC is prepared by using PLD technique. The YBCO film is grown on different substrates such as SrTiO₃ (STO), LaAlO₃ (LAO), and MgO with the help of PLD. The grazing incidence X-Ray Diffract meter is use in this process for avoid peaks from the substrates. The YBCO film grown on STO and MgO substrates gives the results high C oriented maximum peak intensity and random orientation. The YBCO film grown on LAO substrates give the high intensity (007) direction present between 20 to 60 degress 2θ range. The XRD patterns of the YBCO film grown on different substrate are recorded. The surface morphology of the YBCO film analysis by using SEM and surfaces of different substrates are give 1000 and 20000 times magnifications. These magnifications give the small size and counting by using the PLD technique.