Bioinformatics is an interdisciplinary field that develops methods and software tools for understanding biological data as an interdisciplinary field of science, bioinformatics combines Computer Science, Biology, Mathematics, and Engineering to analyze and interpret biological data. In other words, Bioinformatics is an academic field that manages information about materials and substances essential for the biological activities of genes, proteins, and RNA. The field of bioinformatics is still at an early stage of development, and more studies are needed to establish its importance and significance; however, it has the potential to develop medical care. Biodiesel is obtained from animal fat and /or vegetable oil by transesterification with methanol, has attracted significant attention recently as a renewable, biodegradable and nontoxic fuel. Brain tumors are devastating owing to the high fatality rate and the devastating impact on life qualities of patients. Recent advancement of comparative transcriptome profiling tools and mouse genetic models has greatly deepened our understanding of the developmental origins of these tumors. This paper describes the past, present and future possibility of Translational Bio-informatics. Bioinformatic methods are useful for forensic DNA analyses.