The present study deals with the ichthyospecies diversity of Panishala Beel of Cooch Behar District of West Bengal, India. The Panishala Beel lies in the geographical ordinates between 26^o16´14´´N latitude 89^o31´46´´E longitudes. A survey was conducted on composition of fish fauna of the Panishala Beel from December, 2016 to November, 2017. During the study, 39 fish species belonging to 28 genera under 16 families from 8 orders was recorded. According to IUCN red list, 1 species is belongs to vulnerable status, 1 species is in data deficient category, 30 species are least concern and other 7 species are not evaluated. The 16 different families recorded in the study are Belonidae, Cyprinidae, Cobitidae, Aplocheilidae, Notopteridae, Ambassidae, Anabantidae, Channidae, Nandidae, Osphronemidae, Bagridae, Clariidae, Heteropneustidae, Mastacembelidae, Synbranchidae and Tetrodontidae. The present study shows that Cyprinidae is the most dominant family with 13 number of species contributing about 33.33% of the 16 recorded family followed by Channidae (10.27%). The study also reveals that the Panishala Beel is diverse in its fish composition.