This study was conducted to determine the relationship between water quality parameters, depth of water and fish distribution in Cicir, Perpek and Terengganu rivers of Kenyir Lake, Malaysia. Water temperature and dissolved oxygen were measured using an S-C-T meter and YSI-meter at depths of 0, 2.5, 5 and 10m at 0900, 1800 and 2200 hours. Fish were sampled using gillnets with different meshsizes of 5.08, 6.35, 7.62 and 10.16cm. Dissolved oxygen and water temperature recorded a higher value in upper layer and decreased in the deeper layer. Deepest water (10 m) recorded 1194 individuals while surface water layer recorded 313 individuals. There were 2330 individuals from 13 species caught in this study. Hampala macrolepidota was the most dominant (879 individuals) and followed by Cyclocheilichthys apogon (376 individuals). The least dominant species was Mastacembelus armatus (1 individual). Overall, this study showed that fish density has inverse relationship with water quality parameters.