Snuff is a smokeless tobacco made from ground or pulverized tobacco leaves. It is inhaled or sniffed into the nasal cavity delivering a swift hit of nicotine and a lasting flavored scent. The microbial contamination of snuff sold in selected markets in Yenagoa, Bayelsa State, Nigeria was carried out using standard microbiological techniques. The total bacterial counts shows highest in Swali market and least in Tombia market which was 6.2 x 10⁴, 5.5 x 10⁴, 5.3 x 10⁴ and 4.8x10⁴cfu/g for Swali, Kpansia, Opolo, and Tombia markets respectively. While total fungal counts was highest in Kpansia market and least in Tombia market which was 4.2 x 10⁴, 2.0 x 10⁴, 1.9 x 10⁴ and 1.2 x 10⁴ cfu/g for Kpansia, Opolo, Swali and Tombia markets respectively. The bacteria characterization and identification shows the presence of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Micrococcus luteus and Escherichia coli. While those of fungi shows the presence of Aspergillus niger, Aspergillus fumigatus, Geotrichum albidum, Penicillium oxalicum and Geotrichum candidiasis. The snuffs sold in these markets were contaminated by potentially pathogenic microorganisms. Good manufacturing process and improvement in the sanitary conditions of the markets will help in reducing these contaminations.