Zooplanktons are freshwater habitats and found cosmopolitan in nature. They are integral part of aquatic food web and contribute significantly to aquatic biological productivity in freshwater ecosystems. It is helpful in evaluating the ecological status of the aquatic habitats as they are important nutritive level as well as used for determining the health of an aquatic ecosystem. The present study reveals that the study site, the Matiyari dam is rich in biodiversity. 18 taxa of Zooplankton were recorded from Matiyari dam which comprised of 5 species of Copepods, 6 species of Rotifera, 4 species of Cladocera and 3 species of Protozoa and one species of Ostracoda. High diversity of Copepoda and Rotifera indicates the presence of suspended material in the water body may lead to the degradation of the perennial water body. The present investigation may help the authorities for conservation and management of these water bodies.