The present paper deals the Phytochemical and antimicrobial study of Hemidesmus indicus L. medicinal plant. The solvents used for the extraction of plant roots were ethanol, methanol and distilled water. The in-vitro antibacterial activity was performed by agar well diffusion method. The most susceptible Gram-Positive and Gram-negative bacteria were tested. The extracts of plant Hemidesmus indicus (L.) inhibited the growth of the bacterial strains investigated. The most active extracts were compared with the standard antibiotics, penicillin, Streptomycin and Ampicillin 100mg/disc). The results obtained in the present study suggest that preliminary phytochemical analysis detected the presence of Alkaloids, Aminoacid, Flavonoids Saponins and Tannins. The Hemidesmus indicus (L.) could be used in treating diseases caused by the test organisms. The results are discussed in detail.