Antibiotic resistance is one of the biggest threats to global health, food security, and development today. Thus, the study was primarily carried out to investigate the presence of multi drug resistant bacteria in different sites like dressing materials, OT table, air, floor, labour wards, bed sheets and instruments of the Operation Theater, etc. of hospital collected from Chidambaram Government Hospital, Cuddalore, Tamil Nadu, India. Antibiotic resistant bacteria were identified by Kirby Bauer diffusion technique and are categorized as sensitive or resistant based on the zone of inhibition of antibiotics. 48 bacterial strains were selected and found Pseudomonas was the predominant organism in Chidambaram GH. 7 strains were found to exhibit antibiotic resistance. Among them, 2 strains were found to be multidrug resistant and were identified as Pseudomonas aeruginosa (Resistant to amoxicillin, streptomycin, kanamycin and Norfloxacin) and Klebsiella pneumonia (Resistant to amoxicillin, streptomycin, kanamycin and gentamicin). Thus, Antibiotic resistance is accelerated by the misuse and overuse of antibiotics, as well as poor infection prevention and control. Steps should be taken to reduce the impact and limit the spread of resistance.