An ionic liquid modified magnetic sorbent was prepared and used in dispersive solid-phase extraction method to isolate berberine. The adsorption efficiency was optimized under various conditions. In berberine standard ethanol solution, the modified magnetic sorbent obtained the highest adsorption amount within 7 h at 30oC. When the sorbent and method were applied in isolation of berberine from natural plant extracted solution, the LOD (4.8×10-4 mg/mL), LOQ (1.6×10-3 mg/mL), the recoveries (87.8-99.6%) and RSDs (8.6-9.9%) proved the accuracy of this sorbent.