This study investigated the concentrations of some heavy metals in surface water of the Kolo Creek, Ogbia axis, Bayelsa State, Nigeria. The study was carried out by collecting samples from four different stations within the creek. The heavy metals concentrations were analyzed using atomic absorption spectrophotometers (AAS) after necessary laboratory processes has been carried out. The concentrations of heavy metals obtained after examination were in the range 0.278±0.10 to 2.461±0.15mg/L for Pb, not detected to1.427±0.13mg/L for Cd, 1.589±0.12 to 4.856±1.01mg/L for Ni, 1.682±0.17 to 4.581±0.56mg/L for Cr, 2.468±0.50 to 6.306±1.23mg/L for Cu, 4.503±1.02 to 7.116±1.53mg/L for Zn, not detected to 1.141±0.57mg/L for As, not detected to 0.472±0.21mg/L for Co and 2.138±0.16 to 6.814±1.20mg/L for Fe. The mean concentrations of heavy metals in the creek were in the order Zn > Cu > Fe > Ni > Cr >Pb > Cd > As > Co. The concentrations of the studied heavy metals in water samples were above WHO maximum permissible limits for potable water. The pollution indices used in measuring the extent of contamination of the surface water by heavy metals indicated different levels of contamination or pollution. The study has shown that the surface water of Kolo Creek has reached an alarming stage due to the high occurrence of heavy metals and therefore urgent steps must be taken to forestall any impending danger that could pose severe threat to the aquatic ecosystem.