Hepatoprotective activities of phyto-constituents are widely documented. Galangin's radical scavenging ability has been extensively researched. The current study sought to determine Galangin's ability to protect rats from liver harm sustained by isoniazid and rifampicin. Wistar rats were used in this experiment. Hepatocellular damage was produced by a 21-day course of isoniazid (100 mg/kg) and rifampicin (100 mg/kg). Galangin doses used were 50, 100, and 200 mg/kg body weight. At the completion of the research, blood was collected and testing methods were performed to determine the antioxidant level. Galangin treatment (50,100, and 200 mg/kg body weight) restored AST, ALT, and ALP levels.SOD and catalase levels were also restored as a result of the medication. TNF-Α-, IL-1, IL-6, MDA, and nitric oxide release all reduced. The results show that Galangin has a considerable hepatoprotective effect. Galangin's hepatoprotective impact might be achieved via its scavenging free radicals and cytokine regulating properties.